Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem Service Inc Tris(1,3-dichloroisopropyl)phosphate | 13674-87-8 | MFCD00083121 | 250mg
Tris(1,3-dichloroisopropyl)phosphate | MW: 430.88 | 13674-87-8 | MFCD00083121 | 250mg
Mitegen HEPES/ NaOH, 1M, pH7.5, 250 ml, CAS # 7365-45-9
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Sigma Aldrich Fine Chemicals Biosciences CHES | 103-47-9 | MFCD00003835 | 1kg
CHES | Purity: 99% | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 1kg
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4) - 4L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration.
Rockland Immunochemicals 1.0 M Tris HCl pH 7.4 - MB-002
Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Tris HCl solution (tris(hydroxymethyl)aminomethane hydrochloride) is useful for stabilization of buffer formulations and electrophoresis of biological molecules. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
United States Biological Corporation Tris Base Ultrapure, 77-86-1, MFCD00004679, 5kg
Molecular Formula C₄H₁₁NO₃, Purity ≥99.9%, Molecular Biology Grade, Molecular Weight 121.14, Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture | 60-00-4 | MFCD00003541 | 500g
Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500g
Sigma Aldrich Fine Chemicals Biosciences MOPS BioUltra, for molecular biology, >=99.5% (T) | 1132-61-2 | MFCD00006183 | 500G
MOPS BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 500G
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 100G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 100G
![Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B78000-1000.0.JPG-250.jpg)
Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kilogram
- Substance Name: 1,3-Bis (TRIS(hydroxymethyl)methylamino)propane
- CAS Number: 64431-96-5
- Molecular Formula: C11H26N2O6
- Molecular Weight: 282.3
![Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92060-25.0.JPG-250.jpg)
Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams
- Substance Name: 3-(N-Morpholino)2-hydroxypropanesulfonic acid
- CAS Number: 68399-77-9
- Molecular Formula: C7H15NO5S
- Molecular Weight: 225.3
Teknova 10mM Tris-HCl Solution, pH 8.0. 50ml. Sterile. 25 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Quality Biological Inc Tris HCl (1 M), pH 7.5, 100 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months