Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
General Laboratory Products Phosphate Buffered Water, 9mL 100/pk
Phosphate Buffered Water - 9ml, 16x125mm polycarbonate tube, 100 per case. For use as a diluent in microbial limit testing of food and water.
Abcam E x traction Buffer 5 x PTR, 50ML
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
The product is subject to the following: Abcam Restricted Use Statement
Rockland Immunochemicals 50X Tris-Acetate-EDTA (TAE) (2.0 M Tris-Acetate 0.05 M EDTA pH 8.3) - MB-020
Tris-Acetate-EDTA (TAE) is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. TAE suitable for multiplemolecular biology applications including agarose electrophoresis for the separation of nucleic acids such as DNA and RNA.
![eMolecules 39946-25-3 | 2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925](https://assets.fishersci.com/TFS-Assets/CCG/product-images/NC3454793.JPG-250.jpg)
eMolecules 39946-25-3 | 2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925
2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | 39946-25-3 | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925
Gold Biotechnology Inc MES Free Acid Monohydrate, Ultra Pure 100 g
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography.
Abcam Native lysis Buffer, 15ML
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
The product is subject to the following: Abcam Restricted Use Statement
HORIZON DISCOVERY BIOSCIENCES LIMITED 10MM TRIS-HCL BUFFER PH 7.4
NC2372517 10MM TRIS-HCL BUFFER PH 7.4
Abcam Mammalian Cell Lysis Buffer 5X (ab179835)
Mammalian Cell Lysis Buffer 5X (ab179835)
The product is subject to the following: Abcam Restricted Use Statement
TRIBIOSCIENCE INC Inoue Transformation Buffer, 100ML
Inoue Transformation Buffer is used for the preparation of high-efficient competent cells. This product is based on the Inoue method for the preparation and transformation of E. Coli component cells.
Meso Scale Discovery MSD GOLD Read Buffer B | 200mL
MSD GOLD Read Buffer B, Read buffer is an essential component of MSD immunoassays: it is required for the generation of electrochemiluminescence (ECL) signals. MSD GOLD Read Buffer B was optimized for use in all MSD assays and can be used as a replacement for MSD GOLD Read Buffer A and MSD Read Buffer T (4x). MSD GOLD Read Buffer B is also recommended for assays that measure whole cell, vesicles, or other membrane-containing analytes. MSD GOLD Read Buffer B has stringent quality control release criteria that ensure lot-to-lot reproducibility. The specifications are designed to meet the requirements of critical assays and longitudinal studies.
