Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld EGTA 0.5M, pH 8.0, DNase and RNase Free, 50 mL
EGTA Buffer is of a 0.5M concentration and pH of 8.0 that is DNase and RNase free. EGTA Buffer is a metal chelating agent specific for calcium ions. EGTA Buffer is commonly used in tissue culture media and biological buffers.
Worthington Biochemical Corporation EARK=LE'S BUFFER VIAL
Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.
IBI SCIENTIFIC 3P 10X TBE Buffer, 1 liter: Superior Buffering Capacity for Electrophoresis Applications
TBE (Tris / Boric Acid / EDTA) buffer is a high-performance solution designed to offer superior buffering capacity compared to TAE. With its enhanced ability to maintain pH stability over extended electrophoresis runs, TBE is the preferred choice for DNA sequencing, gel electrophoresis, and other applications requiring high-resolution separation. This buffer eliminates the need for recirculation in most electrophoresis protocols, making it more efficient and reliable for laboratories conducting extended gel runs.
Sigma Aldrich Fine Chemicals Biosciences HANKS BALANCED SALT SOLUTION
NC3660344 HANKS BALANCED SALT SOLUTION
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 4L
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications. This product functions as wash buffer in Western blotting and immunoassays, and is formulated in a 10X concentration. Dilute to desired concentration.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffer Solution | 14265-44-2 | MFCD00145108 | 1 gal
Phosphate Buffer Solution | Mol Wt: 94.97 | 14265-44-2 | MFCD00145108 | 1 gal
Sciencell Research Laboratories DULBECCO'S PHOSPHATE-BUFFERED
Dulbecco's Phosphate-Buffered Saline 500 ml